Creatine Kinase-MB (CK-MB / CKM) mRNA LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

Creatine Kinase-MB (CK-MB / CKM) mRNA LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

$2,520.00

Pre-optimized LAMP primer system targeting Creatine Kinase-MB (CKM) transcripts for fluorescence-based isothermal amplification. Designed for research-use myocardial injury and cardiac biomarker studies and integration into rapid isothermal assay platforms. For Research Use Only (RUO).

Category:

Description

The ProbeSeq Creatine Kinase-MB (CK-MB / CKM) mRNA LAMP Primer Set is a ready-to-use six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting CKM transcripts. This product supports research-use molecular cardiology applications, including myocardial injury studies, cardiac enzyme expression research, and integration into rapid isothermal amplification systems.

Creatine Kinase-MB (CK-MB) is a cardiac-associated isoenzyme historically used as a biomarker of myocardial infarction and cardiac muscle injury. While commonly assessed at the protein level, transcript-level detection of CKM provides additional insight in experimental cardiac models, ischemia-reperfusion studies, and molecular injury pathway investigations.

This primer system is structured to promote stable amplification kinetics under isothermal conditions while supporting consistent fluorescence signal development. Primer architecture is optimized to minimize non-specific amplification artifacts and to ensure compatibility with fluorescence-based detection systems.

What’s Included

Each set contains a complete LAMP primer architecture:

  • F3 and B3 outer primers

  • FIP and BIP inner primers

  • LF and LB loop primers

Primers are individually labeled and supplied with sequence documentation for research traceability and workflow integration.

Target Strategy

The primer system targets conserved regions within CKM transcripts selected for:

  • Cardiac tissue relevance

  • Transcript specificity

  • Minimal homology to non-target human sequences

  • Compatibility with reverse transcription–LAMP (RT-LAMP) workflows

Target selection prioritizes assay robustness and integration into fluorescence-based monitoring platforms.

Product Format

Available in two configurations:

Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer

Solution format
• 500 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer

Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom bulk quantities are available for cardiovascular research programs.

Performance Orientation

Designed for compatibility with:

  • Reverse transcription–LAMP (RT-LAMP) workflows

  • Intercalating dye fluorescence monitoring

  • Real-time isothermal-capable instruments

  • Portable fluorescence readers

  • Microfluidic and cartridge-integrated amplification systems

Typical reaction temperature range: 60–65 °C. RNA-based applications require inclusion of reverse transcriptase activity in the reaction system.

Quality Control

Each batch undergoes:

  • Sequence verification

  • Mass confirmation

  • Concentration validation

  • Manufacturing quality control review

  • Certificate of Analysis (CoA) available upon request.

Storage

Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended conditions.
Avoid repeated freeze–thaw cycles for solution format.

Applications

  • Myocardial infarction research

  • Cardiac muscle injury studies

  • Molecular cardiology research

  • Rapid isothermal assay prototyping

  • Biosensor and microfluidic cardiovascular integration research

Important Notice

For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.

Related products