Description
The ProbeSeq Creatine Kinase-MB (CK-MB / CKM) mRNA LAMP Primer Set is a ready-to-use six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting CKM transcripts. This product supports research-use molecular cardiology applications, including myocardial injury studies, cardiac enzyme expression research, and integration into rapid isothermal amplification systems.
Creatine Kinase-MB (CK-MB) is a cardiac-associated isoenzyme historically used as a biomarker of myocardial infarction and cardiac muscle injury. While commonly assessed at the protein level, transcript-level detection of CKM provides additional insight in experimental cardiac models, ischemia-reperfusion studies, and molecular injury pathway investigations.
This primer system is structured to promote stable amplification kinetics under isothermal conditions while supporting consistent fluorescence signal development. Primer architecture is optimized to minimize non-specific amplification artifacts and to ensure compatibility with fluorescence-based detection systems.
What’s Included
Each set contains a complete LAMP primer architecture:
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F3 and B3 outer primers
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FIP and BIP inner primers
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LF and LB loop primers
Primers are individually labeled and supplied with sequence documentation for research traceability and workflow integration.
Target Strategy
The primer system targets conserved regions within CKM transcripts selected for:
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Cardiac tissue relevance
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Transcript specificity
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Minimal homology to non-target human sequences
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Compatibility with reverse transcription–LAMP (RT-LAMP) workflows
Target selection prioritizes assay robustness and integration into fluorescence-based monitoring platforms.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• 500 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer
Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom bulk quantities are available for cardiovascular research programs.
Performance Orientation
Designed for compatibility with:
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Reverse transcription–LAMP (RT-LAMP) workflows
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Intercalating dye fluorescence monitoring
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Real-time isothermal-capable instruments
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Portable fluorescence readers
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Microfluidic and cartridge-integrated amplification systems
Typical reaction temperature range: 60–65 °C. RNA-based applications require inclusion of reverse transcriptase activity in the reaction system.
Quality Control
Each batch undergoes:
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Sequence verification
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Mass confirmation
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Concentration validation
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Manufacturing quality control review
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Certificate of Analysis (CoA) available upon request.
Storage
Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended conditions.
Avoid repeated freeze–thaw cycles for solution format.
Applications
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Myocardial infarction research
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Cardiac muscle injury studies
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Molecular cardiology research
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Rapid isothermal assay prototyping
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Biosensor and microfluidic cardiovascular integration research
Important Notice
For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.
