Description
Product Overview
The ProbeSeq Thrombin Cleavable PeptideProbe™ System provides sequence-optimized peptide substrates for selective detection of thrombin activity, a central enzyme in blood coagulation and hemostasis regulation. The system enables quantitative measurement of thrombin-mediated cleavage associated with clot formation, cardiovascular disease, and coagulation disorders.
This product supports development of enzyme-based diagnostic assays, biosensor platforms, and point-of-care coagulation monitoring technologies. Peptide probes are engineered for high cleavage specificity, rapid enzymatic response, and stable signal generation across fluorescence, electrochemical, and microfluidic detection formats.
The system is compatible with molecular diagnostics workflows, lab-on-chip devices, and multiplex biomarker detection platforms.
Peptide Probe Technology Advantages
Peptide cleavage probes enable direct measurement of enzyme activity rather than protein concentration alone, providing functional insight into coagulation status. Compared to antibody-based detection, peptide probes offer faster kinetics, tunable specificity, strong biosensor compatibility, and reproducible signal response.
Molecular Detection Mechanism
The peptide probe contains a thrombin-specific cleavage sequence that is selectively hydrolyzed by active thrombin. Enzymatic cleavage produces a measurable signal change through reporter release, fluorescence activation, or electrochemical response.
The system supports real-time monitoring of coagulation enzyme activity and quantitative analysis of hemostatic function.
Target Design Strategy
Peptide sequences are optimized for selective recognition and cleavage by thrombin.
Design optimization prioritizes:
High substrate specificity for thrombin
Rapid cleavage kinetics
Minimal non-specific protease interaction
Stable peptide structure in biological samples
Compatibility with reporter conjugation
Reproducible enzymatic response
Custom cleavage sequences available.
Assay Workflow Integration
The PeptideProbe™ system supports workflows including sample incubation, enzymatic cleavage reaction, and signal detection.
The assay integrates with:
Fluorescence detection platforms
Electrochemical biosensors
Microfluidic diagnostic devices
Point-of-care testing systems
Multiplex protease detection assays
Automated clinical analysis workflows
System Components
Sequence-validated thrombin peptide probes
Recommended reaction conditions
Reporter compatibility guidance
Buffer and handling instructions
Signal detection protocols
Custom probe formats available upon request.
Platform Compatibility
Fluorescence detection systems
Electrochemical sensing platforms
Microfluidic diagnostic cartridges
Lab-on-chip technologies
Lateral-flow detection systems
Clinical biomarker research workflows
Performance Characteristics
High cleavage specificity, rapid enzymatic response, low background signal, strong reporter compatibility, stable performance in biological samples, and scalable assay integration.
Technical Specifications
Detection target: Thrombin protease activity
Probe format: Synthetic cleavable peptide substrate
Conjugation options: Fluorophore / quencher / electrochemical tag / linker ready
Purity: ≥95%
Format: Lyophilized or solution
Storage: –20 °C recommended
Quality Assurance
Sequence verification, purity validation, functional cleavage testing, and manufacturing quality review performed for each batch.
Research Applications
Coagulation and hemostasis research
Cardiovascular biomarker detection
Point-of-care coagulation testing
Biosensor engineering
Microfluidic molecular diagnostics
Drug monitoring studies
Storage and Handling
Store at –20 °C in dry conditions. Avoid repeated freeze–thaw cycles. Reconstitute in recommended buffer before use.
For Research Use Only (RUO).
