Description
Surface-functional biosensor recognition ligand designed for selective detection of Tumor Necrosis Factor Alpha (TNF-α), supplied as a thiol-modified DNA aptamer for direct immobilization on gold and thiol-reactive sensor surfaces. Engineered for controlled probe orientation, high binding specificity, and stable integration into electrochemical, optical, and microfluidic biosensor platforms. For Research Use Only (RUO).
Product Description
The ProbeSeq Tumor Necrosis Factor Alpha BiosensorProbe™ is a thiol-functionalized DNA aptamer developed for selective recognition and capture of TNF-α, a key pro-inflammatory cytokine involved in immune activation, autoimmune disease progression, and systemic inflammatory responses. The probe contains a terminal sulfhydryl (–SH) functional group enabling covalent immobilization on gold and thiol-reactive interfaces while preserving aptamer conformation and binding activity.
TNF-α is a ~17 kDa cytokine primarily produced by activated macrophages and immune cells and plays a central role in inflammatory signaling pathways. Elevated TNF-α levels are associated with rheumatoid arthritis, inflammatory bowel disease, sepsis, cancer progression, and cytokine storm responses. This BiosensorProbe™ enables stable surface immobilization and high-affinity target capture in complex biological environments.
Recognition Mechanism
Target recognition is mediated by sequence-engineered single-stranded DNA that folds into a defined three-dimensional structure upon interaction with TNF-α. Binding occurs through hydrogen bonding, electrostatic interactions, and molecular shape complementarity, producing stable surface-associated complexes suitable for label-free and signal-amplified detection.
Specificity and Selectivity
High molecular specificity toward TNF-α with minimal cross-reactivity to related cytokines including IL-1β, IL-6, and IL-8. Sequence-specific recognition enables selective detection in serum, plasma, and cell culture samples with reduced non-specific adsorption.
Molecular Format and Functionalization
Synthetic single-stranded DNA aptamer with terminal thiol (–SH) functional group for controlled surface coupling. High chemical purity ensures preserved structural folding, functional group reactivity, and reproducible biosensor fabrication.
Surface Immobilization Chemistry
Direct chemisorption on gold electrodes via metal–thiol bonding and compatibility with maleimide-functionalized surfaces and thiol-reactive coatings. Immobilized probes maintain structural integrity and target accessibility under physiological assay conditions.
Compatible Sensor Surfaces
Gold electrodes and nanostructured gold substrates
Gold nanoparticle-modified interfaces
Thiol-reactive polymer coatings
Maleimide-functionalized surfaces
Electrochemical and impedance sensors
Microfluidic biosensor platforms
Surface plasmon resonance chips
Performance Characteristics
High specificity toward TNF-α
Stable surface immobilization
Low background binding
Reproducible surface coverage
Compatible with multiplex inflammatory panels
Suitable for label-free detection
Stability
Chemically stabilized to preserve aptamer folding and thiol reactivity under recommended storage and assay conditions. Maintains binding activity after surface immobilization and repeated assay cycles.
Storage
Store at −20°C in supplied buffer. Avoid repeated freeze–thaw cycles and prolonged exposure to oxidizing conditions. Stable for at least 12 months when stored as recommended.
Applications
Inflammation monitoring
Cytokine signaling research
Sepsis and immune response studies
Autoimmune disease biomarker detection
Electrochemical biosensor development
Wearable sensing platforms
Multiplex biomarker panels
