Description
Product Overview
The ProbeSeq CXCR4 FRAPProbe™ System provides fluorescence-labeled probes for quantitative measurement of C-X-C chemokine receptor type 4 (CXCR4) dynamics in live cells. The system enables analysis of receptor diffusion, ligand-induced activation, receptor clustering, and membrane trafficking associated with chemokine signaling pathways.
This assay supports mechanistic investigation of immune cell migration, cancer metastasis, stem cell homing, and receptor-mediated signaling processes. Probe design ensures high photostability, controlled photobleaching performance, and reproducible fluorescence recovery for quantitative FRAP measurements.
The system is compatible with confocal microscopy, live-cell imaging platforms, microfluidic cell culture systems, and immune cell interaction models.
FRAP Technology Advantages
FRAP enables real-time measurement of receptor mobility and signaling dynamics within living cells. Unlike static binding assays, FRAP directly quantifies receptor diffusion behavior, membrane organization, and ligand-induced trafficking under physiological conditions. This supports mechanistic studies of chemokine signaling, immune response regulation, and therapeutic targeting.
Molecular Detection Mechanism
Fluorescent CXCR4 probes selectively bind receptor molecules expressed on the cell membrane. A defined region is photobleached using a focused laser pulse, followed by monitoring fluorescence recovery as unbleached receptors diffuse into the region.
Recovery kinetics provide quantitative measurements of receptor diffusion coefficients, mobile fraction, and membrane trafficking behavior. The system supports analysis of ligand binding effects, receptor internalization, and signaling-induced membrane reorganization.
Target Design Strategy
Probe design targets extracellular CXCR4 domains while preserving native receptor activity.
Design optimization prioritizes:
High binding specificity to CXCR4
Minimal non-specific membrane interaction
Strong photostability during imaging
Controlled photobleaching kinetics
Compatibility with live-cell conditions
Stable fluorescence recovery performance
Antibody-, ligand-, or nanobody-based probe formats available.
Assay Workflow Integration
The FRAPProbe™ system supports workflows including live-cell labeling, fluorescence imaging, targeted photobleaching, and quantitative recovery analysis.
The assay integrates with:
Confocal microscopy systems
High-content imaging platforms
Microfluidic immune cell models
Cell migration and chemotaxis assays
Organ-on-chip platforms
Quantitative image analysis software
System Components
Fluorescent CXCR4-binding probes
Recommended imaging and photobleaching parameters
Control reagents for calibration
Buffer and labeling guidelines
Data analysis recommendations
Custom probe formats available upon request.
Platform Compatibility
Confocal microscopy systems
Live-cell imaging platforms
High-content screening instruments
Microfluidic cell culture systems
Organ-on-chip devices
Immunology and cancer research workflows
Performance Characteristics
High fluorescence signal intensity, strong photostability, reproducible photobleaching response, rapid recovery measurement capability, low background binding, and quantitative receptor mobility analysis.
Technical Specifications
Detection target: CXCR4 receptor
Probe format: Fluorescent antibody / ligand / nanobody conjugate
Fluorophore options: GFP-compatible, Alexa Fluor, Cy dyes
Imaging compatibility: confocal FRAP systems
Working concentration: assay dependent
Storage: 4 °C or –20 °C depending on probe format
Quality Assurance
Binding specificity validation, fluorescence characterization, photostability testing, functional activity verification, and manufacturing quality review performed for each batch.
Research Applications
Immune cell migration research
Cancer metastasis studies
Chemokine signaling analysis
Stem cell trafficking research
Drug screening and therapeutic targeting
Organ-on-chip immune models
Storage and Handling
Store under recommended temperature conditions protected from light. Avoid repeated freeze–thaw cycles. Use sterile handling for live-cell applications.
For Research Use Only (RUO). Not for diagnostic use.
