Description
Product Overview
The ProbeSeq HER2 FRAPProbe™ System provides fluorescence-labeled probes for quantitative measurement of human epidermal growth factor receptor 2 (HER2 / ERBB2) dynamics in live cells and engineered tissue models. The system enables analysis of receptor diffusion, dimerization, clustering behavior, and ligand- or drug-induced signaling responses.
This product supports cancer biomarker characterization, therapeutic antibody development (e.g., HER2-targeted therapies), receptor trafficking studies, and drug–target engagement analysis. Probe design ensures high photostability, controlled photobleaching behavior, and reproducible fluorescence recovery performance required for accurate FRAP measurements.
The system is compatible with confocal microscopy, high-content imaging systems, organ-on-chip platforms, and microfluidic cell culture models.
FRAP Technology Advantages
FRAP provides real-time, spatially resolved measurement of receptor mobility and membrane transport processes in living cells. Unlike endpoint biochemical assays, FRAP enables direct quantification of receptor diffusion, clustering, and drug-induced changes in membrane organization. This approach supports mechanistic studies of oncogenic signaling and therapeutic response under physiologically relevant conditions.
Molecular Detection Mechanism
Fluorescent HER2 probes selectively bind HER2 expressed on the cell membrane. A defined region is photobleached using a focused laser pulse, followed by monitoring fluorescence recovery as unbleached receptors diffuse into the bleached area.
Recovery kinetics provide quantitative measurements of diffusion coefficients, mobile fraction, and receptor binding dynamics. The system supports analysis of receptor clustering, internalization, and therapeutic antibody interactions.
Target Design Strategy
Probe design targets extracellular HER2 domains while preserving native receptor function and signaling.
Design optimization prioritizes:
High binding specificity to HER2
Minimal non-specific membrane interaction
Strong photostability during repeated imaging
Controlled photobleaching kinetics
Compatibility with live-cell conditions
Stable fluorescence recovery performance
Custom antibody-, ligand-, or nanobody-based probes available.
Assay Workflow Integration
The FRAPProbe™ system supports workflows including live-cell labeling, fluorescence imaging, targeted photobleaching, and quantitative recovery analysis.
The assay integrates with:
Confocal microscopy systems
High-content screening platforms
Microfluidic cell culture devices
Tumor model systems
Drug screening workflows
Quantitative image analysis software
System Components
Fluorescent HER2-binding probes
Recommended imaging and photobleaching parameters
Control reagents for calibration
Buffer and labeling guidelines
Data analysis recommendations
Custom probe formats available upon request.
Platform Compatibility
Confocal microscopy systems
Live-cell imaging platforms
High-content screening instruments
Microfluidic cell culture systems
Organ-on-chip devices
Oncology drug discovery workflows
Performance Characteristics
High fluorescence signal intensity, strong photostability, reproducible photobleaching response, rapid recovery measurement capability, low background binding, and quantitative receptor diffusion analysis.
Technical Specifications
Detection target: HER2 / ERBB2 receptor
Probe format: Fluorescent antibody / ligand / nanobody conjugate
Fluorophore options: GFP-compatible, Alexa Fluor, Cy dyes
Imaging compatibility: confocal FRAP systems
Working concentration: assay dependent
Storage: 4 °C or –20 °C depending on probe format
Quality Assurance
Binding specificity validation, fluorescence characterization, photostability testing, functional activity verification, and manufacturing quality review performed for each batch.
Research Applications
Breast cancer biomarker research
HER2 signaling and receptor dynamics studies
Therapeutic antibody screening
Drug–target engagement analysis
Tumor model characterization
Receptor trafficking studies
Storage and Handling
Store under recommended temperature conditions protected from light. Avoid repeated freeze–thaw cycles. Use sterile handling for live-cell applications.
