Description
Surface-functional biosensor recognition ligand designed for selective detection of Vascular Endothelial Growth Factor A (VEGF-A), supplied as a thiol-modified DNA aptamer for direct immobilization on gold and thiol-reactive sensor surfaces. Engineered for controlled orientation, high binding specificity, and stable integration into electrochemical and microfluidic biosensor platforms. For Research Use Only (RUO).
Product Description
The ProbeSeq Vascular Endothelial Growth Factor A BiosensorProbe™ is a thiol-functionalized DNA aptamer engineered for selective recognition and capture of VEGF-A, a key signaling protein involved in angiogenesis, tumor growth, vascular remodeling, and tissue regeneration. The probe incorporates a terminal sulfhydryl (–SH) functional group enabling controlled covalent attachment to gold and thiol-reactive sensor interfaces while preserving aptamer folding and target-binding activity.
VEGF-A is a homodimeric glycoprotein (~45 kDa) that regulates endothelial cell proliferation, migration, and vascular permeability. Elevated VEGF levels are associated with cancer progression, diabetic retinopathy, cardiovascular disease, and inflammatory conditions. The BiosensorProbe™ enables stable surface immobilization and sensitive target detection in complex biological samples.
Recognition Mechanism
Target recognition is mediated by a sequence-engineered single-stranded DNA aptamer that adopts a defined three-dimensional structure upon interaction with VEGF-A. Binding occurs through hydrogen bonding, electrostatic interactions, and molecular shape complementarity, producing stable surface-bound complexes suitable for label-free and signal-amplified detection.
Specificity and Selectivity
The VEGF-A BiosensorProbe™ demonstrates high specificity toward VEGF-A with minimal cross-reactivity toward related growth factors such as PDGF and FGF family proteins. Structural epitope recognition enables selective detection in serum, plasma, and cell culture media with reduced background signal.
Molecular Format and Functionalization
Synthetic single-stranded DNA aptamer containing a terminal thiol (–SH) functional group for controlled surface immobilization. The probe is purified to high chemical purity and stabilized to preserve structural folding and functional group reactivity.
Surface Immobilization Chemistry
Direct chemisorption on gold surfaces through metal–thiol bonding and compatibility with maleimide-functionalized interfaces and thiol-reactive coatings. Immobilized probes maintain structural stability and target accessibility under physiological assay conditions.
Compatible Sensor Surfaces
Gold electrodes and nanostructured gold substrates
Gold nanoparticle-modified interfaces
Thiol-reactive polymer coatings
Maleimide-functionalized surfaces
Electrochemical and impedance sensors
Microfluidic biosensor platforms
Surface plasmon resonance chips
Performance Characteristics
High specificity toward VEGF-A
Stable surface immobilization
Low non-specific adsorption
Reproducible surface coverage
Compatible with multiplex biomarker detection
Suitable for label-free sensing
Stability
Chemically stabilized to preserve aptamer conformation and thiol reactivity under recommended storage and assay conditions. Maintains binding activity after immobilization and repeated assay cycles.
Storage
Store at −20°C in supplied buffer. Avoid repeated freeze–thaw cycles and prolonged exposure to oxidizing conditions. Stable for at least 12 months when stored as recommended.
Applications
Cancer biomarker detection
Angiogenesis and vascular biology research
Tumor progression monitoring
Electrochemical biosensor development
Microfluidic diagnostic systems
Wearable biomarker sensing
Multiplex disease panels
