Description
The ProbeSeq Neutrophil Elastase (NE) Aptamer is a high-affinity single-stranded DNA (ssDNA) molecular recognition element engineered for selective binding to human neutrophil elastase, a serine protease released by activated neutrophils during immune responses and neutrophil extracellular trap (NET) formation. This product supports research-use biosensing and diagnostic assay development applications, including NET detection studies, inflammatory response monitoring, and integration into rapid detection platforms.
Neutrophil elastase plays a central role in innate immune defense by degrading microbial proteins and modulating inflammatory signaling pathways. Dysregulated neutrophil elastase activity is associated with sepsis, autoimmune disorders, chronic inflammatory diseases, and cardiovascular pathology. Detection of NE is widely used as an indicator of neutrophil activation and NET formation in translational immunology and inflammation research.
This aptamer provides selective molecular recognition of neutrophil elastase through sequence-defined binding interactions that support high target specificity and strong binding affinity under physiologically relevant conditions. Compared with antibody-based recognition elements, aptamers provide improved chemical stability, reproducible synthesis, and compatibility with electrochemical sensing platforms, surface functionalization strategies, and microfluidic diagnostic systems.
The aptamer sequence is optimized to minimize non-specific adsorption, support stable immobilization on sensing surfaces, and maintain binding performance across diverse assay environments, enabling reliable integration into biosensor development workflows and translational research platforms.
Typical assay workflows use sample input volumes ranging from approximately 5–50 µL depending on platform configuration, surface chemistry, and detection method. The aptamer is compatible with serum, plasma, cell culture media, and buffered biological samples following appropriate assay optimization.
This product supports research programs focused on NET biology, inflammatory disease research, immune response characterization, and biosensor engineering.
What’s Included
Each product contains:
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High-affinity ssDNA aptamer targeting human neutrophil elastase
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Sequence documentation for research traceability
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Reconstitution guidelines and handling instructions
Custom sequence modifications and bulk quantities are available upon request.
Target Strategy
The aptamer is selected against human neutrophil elastase to support:
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High binding specificity to NE protease
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Minimal cross-reactivity with related proteases
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Stable performance under physiological buffer conditions
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Compatibility with biosensor surface immobilization strategies
Target selection prioritizes sensitivity and integration into diagnostic platforms.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• Supplied at defined stock concentration (e.g., 100 µM)
• Provided in nuclease-free water or TE buffer
Custom concentrations and modification options available upon request.
Performance Orientation
Designed for compatibility with:
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Electrochemical biosensors
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NET and inflammation sensing platforms
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Optical and fluorescence-based detection systems
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Surface immobilization workflows
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Microfluidic and lab-on-chip systems
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Point-of-care immune monitoring devices
Quality Control
Each batch undergoes:
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Sequence verification
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Purity confirmation (HPLC purification)
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Applications
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Neutrophil extracellular trap (NET) formation studies
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Inflammatory biomarker detection research
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Immune response monitoring studies
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Sepsis and autoimmune disease research
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Biosensor development and validation
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Microfluidic and point-of-care platform integration
Storage
Store at –20 °C.
Lyophilized aptamers are stable for up to 12 months. Avoid repeated freeze–thaw cycles for solution format.
