Description
The ProbeSeq Cortisol Aptamer is a high-affinity single-stranded DNA (ssDNA) molecular recognition element engineered for selective binding to cortisol, a glucocorticoid hormone that regulates stress response, immune function, metabolism, and circadian rhythm. This product supports research-use biosensing and diagnostic assay development applications, including stress biomarker detection, endocrine monitoring studies, and integration into rapid detection platforms.
Cortisol is widely recognized as a central biomarker of physiological stress and hypothalamic–pituitary–adrenal (HPA) axis activity. Abnormal cortisol levels are associated with chronic stress, metabolic disorders, immune dysfunction, and endocrine diseases. Cortisol monitoring in biological fluids such as saliva, sweat, and blood is increasingly important for stress physiology research and wearable diagnostic development.
This aptamer provides selective molecular recognition of cortisol through sequence-defined binding interactions that support high target specificity and strong binding affinity under physiologically relevant conditions. Compared with antibody-based recognition elements, aptamers provide improved chemical stability, reproducible synthesis, and compatibility with electrochemical sensing platforms, surface functionalization strategies, and microfluidic diagnostic systems.
The aptamer sequence is optimized to minimize non-specific adsorption, support stable immobilization on sensing surfaces, and maintain binding performance across diverse assay environments, enabling reliable integration into biosensor development workflows and translational research platforms.
Typical assay workflows use sample input volumes ranging from approximately 5–50 µL depending on platform configuration, surface chemistry, and detection method. The aptamer is compatible with saliva, sweat, serum, plasma, and buffered biological samples following appropriate assay optimization.
This product supports research programs focused on stress physiology, endocrine regulation, wearable biosensor development, and point-of-care diagnostics.
What’s Included
Each product contains:
-
High-affinity ssDNA aptamer targeting cortisol
-
Sequence documentation for research traceability
-
Reconstitution guidelines and handling instructions
Custom sequence modifications and bulk quantities are available upon request.
Target Strategy
The aptamer is selected against cortisol to support:
-
High binding specificity to cortisol steroid structure
-
Minimal cross-reactivity with related steroid hormones
-
Stable performance under physiological buffer conditions
-
Compatibility with biosensor surface immobilization strategies
Target selection prioritizes sensitivity for hormone monitoring applications.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• Supplied at defined stock concentration (e.g., 100 µM)
• Provided in nuclease-free water or TE buffer
Custom concentrations and modification options available upon request.
Performance Orientation
Designed for compatibility with:
-
Electrochemical biosensors
-
Wearable sensing platforms
-
Hormone detection assays
-
Optical and fluorescence-based detection systems
-
Surface immobilization workflows
-
Microfluidic and lab-on-chip systems
-
Point-of-care endocrine monitoring devices
Quality Control
Each batch undergoes:
-
Sequence verification
-
Purity confirmation (HPLC purification)
-
Concentration validation
-
Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Applications
-
Stress hormone monitoring research
-
Wearable biosensor development
-
Endocrine and metabolic studies
-
Circadian rhythm research
-
Biosensor development and validation
-
Microfluidic and point-of-care platform integration
Storage
Store at –20 °C.
Lyophilized aptamers are stable for up to 12 months. Avoid repeated freeze–thaw cycles for solution format.
