Description
The ProbeSeq D-Dimer Aptamer is a high-affinity single-stranded DNA (ssDNA) molecular recognition element engineered for selective binding to human D-dimer, a fibrin degradation fragment generated during blood clot breakdown. This product supports research-use biosensing and diagnostic assay development applications, including thrombosis detection studies, coagulation monitoring research, and integration into rapid detection platforms.
D-dimer is widely recognized as a clinically validated biomarker of fibrin formation and degradation and is commonly used in studies of venous thromboembolism, pulmonary embolism, disseminated intravascular coagulation, and cardiovascular disease. Elevated D-dimer levels reflect activation of coagulation and fibrinolysis pathways. Sensitive detection of D-dimer is critical for hemostasis research, disease monitoring studies, and development of rapid point-of-care diagnostic technologies.
This aptamer provides selective molecular recognition of D-dimer through sequence-defined binding interactions that support high target specificity and strong binding affinity under physiologically relevant conditions. Compared with antibody-based recognition elements, aptamers provide improved chemical stability, reproducible synthesis, and compatibility with electrochemical sensing platforms, surface functionalization strategies, and microfluidic diagnostic systems.
The aptamer sequence is optimized to minimize non-specific adsorption, support stable immobilization on sensing surfaces, and maintain binding performance across diverse assay environments, enabling reliable integration into biosensor development workflows and translational research platforms.
Typical assay workflows use sample input volumes ranging from approximately 5–50 µL depending on platform configuration, surface chemistry, and detection method. The aptamer is compatible with plasma, serum, and buffered biological samples following appropriate assay optimization.
This product supports research programs focused on coagulation biology, cardiovascular disease research, and biosensor engineering.
What’s Included
Each product contains:
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High-affinity ssDNA aptamer targeting human D-dimer
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Sequence documentation for research traceability
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Reconstitution guidelines and handling instructions
Custom sequence modifications and bulk quantities are available upon request.
Target Strategy
The aptamer is selected against human D-dimer to support:
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High binding specificity to fibrin degradation fragments
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Minimal cross-reactivity with unrelated plasma proteins
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Stable performance under physiological buffer conditions
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Compatibility with biosensor surface immobilization strategies
Target selection prioritizes sensitivity for coagulation biomarker detection.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• Supplied at defined stock concentration (e.g., 100 µM)
• Provided in nuclease-free water or TE buffer
Custom concentrations and modification options available upon request.
Performance Orientation
Designed for compatibility with:
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Electrochemical biosensors
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Coagulation monitoring platforms
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Optical and fluorescence-based detection systems
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Surface immobilization workflows
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Microfluidic and lab-on-chip systems
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Point-of-care thrombosis monitoring devices
Quality Control
Each batch undergoes:
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Sequence verification
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Purity confirmation (HPLC purification)
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Applications
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Thrombosis biomarker detection research
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Coagulation and hemostasis studies
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Cardiovascular disease research
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Biosensor development and validation
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Diagnostic assay development
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Microfluidic and point-of-care platform integration
Storage
Store at –20 °C.
Lyophilized aptamers are stable for up to 12 months. Avoid repeated freeze–thaw cycles for solution format.
