Description
Description
The ProbeSeq Human Epidermal Growth Factor Receptor 2 (HER2 / ERBB2) Aptamer is a high-affinity single-stranded DNA (ssDNA) molecular recognition element engineered for selective binding to HER2, a transmembrane receptor tyrosine kinase involved in cell growth and proliferation signaling pathways. This product supports research-use biosensing and diagnostic assay development applications, including oncology biomarker detection, cancer progression studies, and integration into rapid detection platforms.
HER2 is widely recognized as a clinically validated biomarker in breast cancer and other solid tumors. Overexpression or amplification of HER2 is associated with aggressive tumor growth and therapeutic response monitoring. Sensitive detection of HER2 is critical for oncology research, targeted therapy studies, and development of rapid point-of-care diagnostic technologies.
This aptamer provides selective molecular recognition of HER2 through sequence-defined binding interactions that support high target specificity and strong binding affinity under physiologically relevant conditions. Compared with antibody-based recognition elements, aptamers provide improved chemical stability, reproducible synthesis, and compatibility with electrochemical sensing platforms, surface functionalization strategies, and microfluidic diagnostic systems.
The aptamer sequence is optimized to minimize non-specific adsorption, support stable immobilization on sensing surfaces, and maintain binding performance across diverse assay environments, enabling reliable integration into biosensor development workflows and translational research platforms.
Typical assay workflows use sample input volumes ranging from approximately 5–50 µL depending on platform configuration, surface chemistry, and detection method. The aptamer is compatible with serum, plasma, cell lysates, extracellular vesicles, and buffered biological samples following appropriate assay optimization.
This product supports research programs focused on oncology diagnostics, cancer biomarker research, and biosensor engineering.
What’s Included
Each product contains:
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High-affinity ssDNA aptamer targeting human HER2
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Sequence documentation for research traceability
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Reconstitution guidelines and handling instructions
Custom sequence modifications and bulk quantities are available upon request.
Target Strategy
The aptamer is selected against HER2 receptor to support:
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High binding specificity to HER2 extracellular domain
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Minimal cross-reactivity with related receptor proteins
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Stable performance under physiological buffer conditions
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Compatibility with biosensor surface immobilization strategies
Target selection prioritizes sensitivity for oncology biomarker detection.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• Supplied at defined stock concentration (e.g., 100 µM)
• Provided in nuclease-free water or TE buffer
Custom concentrations and modification options available upon request.
Performance Orientation
Designed for compatibility with:
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Electrochemical biosensors
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Oncology biomarker detection platforms
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Optical and fluorescence-based detection systems
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Surface immobilization workflows
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Microfluidic and lab-on-chip systems
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Point-of-care cancer monitoring devices
Quality Control
Each batch undergoes:
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Sequence verification
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Purity confirmation (HPLC purification)
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Applications
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Breast cancer biomarker detection research
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Oncology diagnostic development
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Cancer progression monitoring studies
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Biosensor development and validation
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Liquid biopsy and extracellular vesicle research
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Microfluidic and point-of-care platform integration
Storage
Store at –20 °C.
Lyophilized aptamers are stable for up to 12 months. Avoid repeated freeze–thaw cycles for solution format.
