Description
The ProbeSeq Lactate Aptamer is a high-affinity single-stranded DNA (ssDNA) molecular recognition element engineered for selective binding to lactate, a central metabolite produced during anaerobic metabolism and cellular energy imbalance. This product supports research-use biosensing and diagnostic assay development applications, including metabolic monitoring studies, tissue hypoxia detection, and integration into rapid detection platforms.
Lactate is widely recognized as a clinically relevant biomarker of tissue oxygen deficiency, metabolic stress, and disease severity. Elevated lactate levels are associated with sepsis, shock, cardiovascular dysfunction, and impaired tissue perfusion. Continuous and rapid lactate monitoring is critical for critical care research, metabolic physiology studies, and development of wearable and point-of-care diagnostic technologies.
This aptamer provides selective molecular recognition of lactate through sequence-defined binding interactions that support high target specificity and strong binding affinity under physiologically relevant conditions. Compared with enzyme-based or antibody-based detection systems, aptamers offer improved chemical stability, reproducible synthesis, and compatibility with electrochemical sensing platforms, surface functionalization strategies, and microfluidic diagnostic systems.
The aptamer sequence is optimized to minimize non-specific adsorption, support stable immobilization on sensing surfaces, and maintain binding performance across diverse assay environments, enabling reliable integration into biosensor development workflows and translational research platforms.
Typical assay workflows use sample input volumes ranging from approximately 5–50 µL depending on platform configuration, surface chemistry, and detection method. The aptamer is compatible with blood, serum, plasma, sweat, interstitial fluid, and buffered biological samples following appropriate assay optimization.
This product supports research programs focused on metabolic monitoring, critical care diagnostics, wearable biosensor development, and biosensor engineering.
What’s Included
Each product contains:
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High-affinity ssDNA aptamer targeting lactate
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Sequence documentation for research traceability
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Reconstitution guidelines and handling instructions
Custom sequence modifications and bulk quantities are available upon request.
Target Strategy
The aptamer is selected against lactate to support:
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High binding specificity to lactate molecules
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Minimal cross-reactivity with related metabolites
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Stable performance under physiological buffer conditions
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Compatibility with biosensor surface immobilization strategies
Target selection prioritizes sensitivity for metabolic monitoring applications.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• Supplied at defined stock concentration (e.g., 100 µM)
• Provided in nuclease-free water or TE buffer
Custom concentrations and modification options available upon request.
Performance Orientation
Designed for compatibility with:
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Electrochemical biosensors
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Wearable sensing platforms
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Metabolic monitoring systems
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Optical and fluorescence-based detection systems
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Surface immobilization workflows
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Microfluidic and lab-on-chip systems
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Point-of-care metabolic monitoring devices
Quality Control
Each batch undergoes:
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Sequence verification
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Purity confirmation (HPLC purification)
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Applications
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Metabolic biomarker detection research
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Sepsis severity monitoring studies
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Tissue perfusion and hypoxia research
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Wearable biosensor development
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Biosensor development and validation
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Microfluidic and point-of-care platform integration
Storage
Store at –20 °C.
Lyophilized aptamers are stable for up to 12 months. Avoid repeated freeze–thaw cycles for solution format.
