Description
The ProbeSeq Neurofilament Light Chain (NfL) Aptamer is a high-affinity single-stranded DNA (ssDNA) molecular recognition element engineered for selective binding to human neurofilament light chain, a structural cytoskeletal protein released during neuronal and axonal injury. This product supports research-use biosensing and diagnostic assay development applications, including neurodegeneration biomarker detection, traumatic brain injury studies, and integration into rapid neurological diagnostic platforms.
Neurofilament light chain is a neuron-specific intermediate filament protein widely recognized as a sensitive biomarker of axonal damage. Elevated NfL levels are associated with multiple sclerosis, Alzheimer’s disease, traumatic brain injury, stroke, and other neurodegenerative disorders. Sensitive detection of NfL is critical for monitoring neuronal injury, disease progression studies, and development of point-of-care neurological testing technologies.
This aptamer provides selective molecular recognition of NfL through sequence-defined binding interactions that support high target specificity and strong binding affinity under physiologically relevant conditions. Compared with antibody-based recognition elements, aptamers provide improved chemical stability, reproducible synthesis, and compatibility with electrochemical sensing platforms, surface functionalization workflows, and microfluidic diagnostic systems.
The aptamer sequence is optimized to minimize non-specific adsorption, support stable immobilization on conductive and polymeric surfaces, and maintain binding performance across diverse assay environments, enabling reliable integration into biosensor development workflows and translational research platforms.
This product supports research programs focused on neurological diagnostics, neurodegenerative disease research, and advanced biosensor technologies.
What’s Included
Each product contains:
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High-affinity ssDNA aptamer targeting human neurofilament light chain
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Sequence documentation for research traceability
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Reconstitution guidelines and handling instructions
Custom sequence modifications and bulk quantities are available upon request.
Target Strategy
The aptamer is selected against human neurofilament light chain to support:
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High binding specificity to neuron-derived NfL protein
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Minimal cross-reactivity with related cytoskeletal proteins
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Stable performance under physiological buffer conditions
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Compatibility with biosensor surface immobilization strategies
Target selection prioritizes sensitivity and integration into diagnostic platforms.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• Supplied at defined stock concentration (e.g., 100 µM)
• Provided in nuclease-free water or TE buffer
Custom concentrations and modification options available upon request.
Performance Orientation
Designed for compatibility with:
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Electrochemical biosensors
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Optical and fluorescence-based detection systems
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Surface immobilization platforms
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Microfluidic and lab-on-chip systems
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Point-of-care neurodiagnostic device development
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Rapid assay prototyping workflows
Quality Control
Each batch undergoes:
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Sequence verification
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Purity confirmation (HPLC purification)
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Applications
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Neurodegenerative disease biomarker detection research
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Multiple sclerosis and Alzheimer’s disease studies
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Traumatic brain injury and stroke research
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Biosensor development and validation
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Neurological diagnostic assay development
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Microfluidic and point-of-care platform integration
Storage
Store at –20 °C.
Lyophilized aptamers are stable for up to 12 months. Avoid repeated freeze–thaw cycles for solution format.
