Description
The ProbeSeq Procalcitonin (PCT) Aptamer is a high-affinity single-stranded DNA (ssDNA) molecular recognition element engineered for selective binding to human procalcitonin, a peptide precursor of calcitonin that is released systemically during bacterial infection and sepsis. This product supports research-use biosensing and diagnostic assay development applications, including infection monitoring, sepsis biomarker detection, and integration into rapid detection platforms.
Procalcitonin is widely recognized as a clinically validated biomarker for systemic bacterial infection and sepsis severity assessment. PCT levels rise rapidly in response to bacterial endotoxins and inflammatory signaling while remaining relatively low in viral infections, making it an important marker for infection differentiation and antibiotic stewardship research. Sensitive detection of PCT is critical for infection monitoring studies, immune response characterization, and development of rapid point-of-care diagnostic technologies.
This aptamer provides selective molecular recognition of procalcitonin through sequence-defined binding interactions that support high target specificity and strong binding affinity under physiologically relevant conditions. Compared with antibody-based recognition elements, aptamers provide improved chemical stability, reproducible synthesis, and compatibility with electrochemical sensing platforms, surface functionalization strategies, and microfluidic diagnostic systems.
The aptamer sequence is optimized to minimize non-specific adsorption, support stable immobilization on sensing surfaces, and maintain binding performance across diverse assay environments, enabling reliable integration into biosensor development workflows and translational research platforms.
Typical assay workflows use sample input volumes ranging from approximately 5–50 µL depending on platform configuration, surface chemistry, and detection method. The aptamer is compatible with serum, plasma, whole blood extracts, and buffered biological samples following appropriate assay optimization.
This product supports research programs focused on infectious disease diagnostics, sepsis research, and biosensor engineering.
What’s Included
Each product contains:
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High-affinity ssDNA aptamer targeting human procalcitonin
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Sequence documentation for research traceability
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Reconstitution guidelines and handling instructions
Custom sequence modifications and bulk quantities are available upon request.
Target Strategy
The aptamer is selected against human procalcitonin to support:
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High binding specificity to PCT peptide structure
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Minimal cross-reactivity with related endocrine peptides
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Stable performance under physiological buffer conditions
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Compatibility with biosensor surface immobilization strategies
Target selection prioritizes sensitivity for infection biomarker detection.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• Supplied at defined stock concentration (e.g., 100 µM)
• Provided in nuclease-free water or TE buffer
Custom concentrations and modification options available upon request.
Performance Orientation
Designed for compatibility with:
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Electrochemical biosensors
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Infection detection platforms
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Optical and fluorescence-based detection systems
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Surface immobilization workflows
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Microfluidic and lab-on-chip systems
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Point-of-care infection monitoring devices
Quality Control
Each batch undergoes:
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Sequence verification
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Purity confirmation (HPLC purification)
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Applications
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Sepsis biomarker detection research
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Bacterial infection monitoring studies
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Immune response and inflammation research
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Biosensor development and validation
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Diagnostic assay development
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Microfluidic and point-of-care platform integration
Storage
Store at –20 °C.
Lyophilized aptamers are stable for up to 12 months. Avoid repeated freeze–thaw cycles for solution format.
