Description
The ProbeSeq Tumor Necrosis Factor-alpha (TNF-α) Aptamer is a high-affinity single-stranded DNA (ssDNA) molecular recognition element engineered for selective binding to human TNF-α, a key pro-inflammatory cytokine involved in immune signaling and inflammatory response pathways. This product supports research-use biosensing and diagnostic assay development applications, including cytokine monitoring, immune response studies, and integration into rapid detection platforms.
Tumor necrosis factor-alpha is a major mediator of inflammation produced primarily by activated macrophages and immune cells. TNF-α regulates immune cell activation, apoptosis, and inflammatory signaling pathways and is strongly associated with autoimmune disorders, sepsis, chronic inflammatory diseases, and cancer progression. Sensitive detection of TNF-α is critical for immune response characterization, disease monitoring research, and development of rapid point-of-care diagnostic technologies.
This aptamer provides selective molecular recognition of TNF-α through sequence-defined binding interactions that support high target specificity and strong binding affinity under physiologically relevant conditions. Compared with antibody-based recognition elements, aptamers offer improved chemical stability, reproducible synthesis, and compatibility with electrochemical sensing platforms, surface functionalization strategies, and microfluidic diagnostic systems.
The aptamer sequence is optimized to minimize non-specific adsorption, support stable immobilization on sensing surfaces, and maintain binding performance across diverse assay environments, enabling reliable integration into biosensor development workflows and translational research platforms.
Typical assay workflows use sample input volumes ranging from approximately 5–50 µL depending on platform configuration, surface chemistry, and detection method. The aptamer is compatible with serum, plasma, cell culture media, and buffered biological samples following appropriate assay optimization.
This product supports research programs focused on inflammatory disease research, immune response monitoring, and biosensor engineering.
What’s Included
Each product contains:
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High-affinity ssDNA aptamer targeting human TNF-α
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Sequence documentation for research traceability
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Reconstitution guidelines and handling instructions
Custom sequence modifications and bulk quantities are available upon request.
Target Strategy
The aptamer is selected against human TNF-α to support:
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High binding specificity to TNF-α cytokine
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Minimal cross-reactivity with related cytokines
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Stable performance under physiological buffer conditions
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Compatibility with biosensor surface immobilization strategies
Target selection prioritizes sensitivity and integration into diagnostic platforms.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• Supplied at defined stock concentration (e.g., 100 µM)
• Provided in nuclease-free water or TE buffer
Custom concentrations and modification options available upon request.
Performance Orientation
Designed for compatibility with:
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Electrochemical biosensors
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Cytokine sensing platforms
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Optical and fluorescence-based detection systems
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Surface immobilization workflows
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Microfluidic and lab-on-chip systems
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Point-of-care inflammation monitoring devices
Quality Control
Each batch undergoes:
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Sequence verification
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Purity confirmation (HPLC purification)
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Applications
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Cytokine monitoring and immune response studies
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Inflammatory biomarker detection research
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Autoimmune disease research
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Sepsis and infection studies
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Biosensor development and validation
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Microfluidic and point-of-care platform integration
Storage
Store at –20 °C.
Lyophilized aptamers are stable for up to 12 months. Avoid repeated freeze–thaw cycles for solution format.
