Description
The ProbeSeq Neurofilament Light Chain (NfL) Nanobody is a recombinant single-domain antibody (VHH) engineered for high-affinity and selective binding to human neurofilament light chain, a structural cytoskeletal protein released into circulation following neuronal damage and axonal injury. This nanobody supports research-use assay development, neurodegeneration biomarker detection, and advanced biosensor integration.
Neurofilament light chain is widely recognized as a sensitive biomarker of neuroaxonal injury and is extensively studied in multiple sclerosis, Alzheimer’s disease, traumatic brain injury, amyotrophic lateral sclerosis, and other neurodegenerative disorders. Elevated NfL levels reflect neuronal damage and disease progression, making sensitive detection critical for neurological research, disease monitoring, and development of rapid diagnostic technologies.
Derived from camelid heavy-chain antibody architecture, this nanobody consists of a single monomeric VHH domain (~15 kDa) providing strong antigen recognition, rapid binding kinetics, and exceptional structural stability. Compared with conventional antibodies, nanobodies offer superior thermal stability, efficient surface immobilization, and reduced steric hindrance, enabling high-performance detection in electrochemical biosensors, microfluidic platforms, and miniaturized diagnostic systems.
Typical assay workflows use sample input volumes ranging from approximately 5–50 µL depending on platform configuration and detection method. The nanobody is compatible with serum, plasma, cerebrospinal fluid, and buffered biological samples following appropriate assay optimization.
What’s Included
Recombinant anti-NfL single-domain VHH nanobody
Sequence and expression documentation for research traceability
Reconstitution and handling guidelines
Custom affinity tags (His-tag, biotin, Fc fusion), labeling options, and bulk production available upon request.
Target Strategy
Engineered for high binding specificity to neurofilament light chain epitopes
Minimal cross-reactivity with related neurofilament proteins
Stable binding under physiological conditions
Compatible with biosensor surface functionalization and immunoassay workflows
Product Format
Lyophilized format
Delivered dry for enhanced stability
Reconstitute in PBS or preferred buffer
Solution format
Supplied in PBS-based storage buffer
Typical concentration range 0.5–1 mg/mL
Tagged variants available upon request.
Performance Orientation
Electrochemical biosensors
Neurodegeneration biomarker detection platforms
Microfluidic and lab-on-chip systems
Surface immobilization workflows
Optical and fluorescence detection platforms
Point-of-care neurological monitoring research devices
Quality Control
Sequence confirmation
Purity validation (SDS-PAGE / chromatography)
Binding verification testing
Concentration determination
Manufacturing quality review
Certificate of Analysis (CoA) available upon request.
Applications
Neurodegeneration biomarker detection research
Multiple sclerosis and Alzheimer’s disease studies
Traumatic brain injury research
Axonal injury monitoring studies
Immunoassay and biosensor development
Microfluidic diagnostic platform integration
Storage
Store at –20 °C for long-term storage or 4 °C for short-term use.
Lyophilized nanobody stable for up to 12 months under recommended conditions. Avoid repeated freeze–thaw cycles.
