Description
The ProbeSeq C-Reactive Protein (CRP) Nanobody is a recombinant single-domain antibody (VHH) engineered for high-affinity and selective binding to human CRP, an acute-phase protein produced by the liver in response to inflammation. This nanobody supports research-use diagnostic assay development, inflammation monitoring studies, and advanced biosensor integration.
C-reactive protein is one of the most extensively studied clinical biomarkers for systemic inflammation, infection, sepsis, and cardiovascular disease risk assessment. CRP levels rapidly increase in response to tissue injury, bacterial infection, and immune activation, making sensitive detection essential for clinical research, therapeutic monitoring, and rapid diagnostic technology development.
Derived from camelid heavy-chain antibody architecture, this nanobody consists of a single monomeric VHH domain (~15 kDa) providing strong antigen recognition, rapid binding kinetics, and exceptional structural stability. Compared with conventional antibodies, nanobodies offer improved thermal stability, efficient surface immobilization, and reduced steric hindrance, enabling high-performance detection in electrochemical biosensors, wearable sensing platforms, and microfluidic diagnostic systems.
Typical assay workflows use sample input volumes ranging from approximately 5–50 µL depending on platform configuration and detection method. The nanobody is compatible with serum, plasma, whole blood, and buffered biological samples following appropriate assay optimization.
What’s Included
Recombinant anti-CRP single-domain VHH nanobody
Sequence and expression documentation for research traceability
Reconstitution and handling guidelines
Custom affinity tags (His-tag, biotin, Fc fusion), labeling options, and bulk production available upon request
Target Strategy
Engineered for high binding specificity to CRP protein epitopes
Minimal cross-reactivity with related plasma proteins
Stable binding under physiological conditions
Compatible with biosensor surface functionalization and immunoassay workflows
Product Format
Lyophilized format
Delivered dry for enhanced stability
Reconstitute in PBS or preferred buffer
Solution format
Supplied in PBS-based storage buffer
Typical concentration range 0.5–1 mg/mL
Tagged variants available upon request
Performance Orientation
Electrochemical biosensors
Inflammation detection assays
Wearable sensing platforms
Microfluidic and lab-on-chip systems
Optical and fluorescence detection platforms
Point-of-care inflammation monitoring research devices
Quality Control
Sequence confirmation
Purity validation (SDS-PAGE / chromatography)
Binding verification testing
Concentration determination
Manufacturing quality review
Certificate of Analysis (CoA) available upon request
Applications
Inflammation biomarker detection research
Sepsis and infection monitoring studies
Cardiovascular risk biomarker research
Immunoassay and biosensor development
Microfluidic diagnostic platform integration
Point-of-care monitoring technology research
Storage
Store at –20 °C for long-term storage or 4 °C for short-term use.
Lyophilized nanobody stable for up to 12 months under recommended conditions. Avoid repeated freeze–thaw cycles.
