Description
The ProbeSeq Interleukin-6 (IL-6) Nanobody is a recombinant single-domain antibody (VHH) engineered for high-affinity and selective binding to human IL-6, a central mediator of inflammatory and immune signaling pathways. This nanobody supports research-use assay development, cytokine detection studies, and advanced biosensor integration.
Interleukin-6 is a clinically relevant biomarker associated with systemic inflammation, infection, autoimmune disease, and cytokine storm responses. Elevated IL-6 levels are widely studied in sepsis, chronic inflammatory disorders, cancer progression, and immune activation. Sensitive detection of IL-6 is critical for immune response monitoring, therapeutic research, and development of rapid diagnostic technologies.
Derived from camelid heavy-chain antibody architecture, this nanobody consists of a single monomeric VHH domain (~15 kDa) providing strong antigen recognition, rapid binding kinetics, and exceptional structural stability. Compared with conventional antibodies, nanobodies offer superior thermal stability, reduced steric hindrance, and efficient surface immobilization, enabling high-performance detection in electrochemical biosensors, microfluidic platforms, and miniaturized diagnostic systems.
Typical assay workflows use sample input volumes ranging from approximately 5–50 µL depending on platform configuration and detection method. The nanobody is compatible with serum, plasma, cell culture supernatants, and buffered biological samples following appropriate assay optimization.
What’s Included
Recombinant anti-IL-6 single-domain VHH nanobody
Sequence and expression documentation for research traceability
Reconstitution and handling guidelines
Custom affinity tags (His-tag, biotin, Fc fusion), labeling options, and bulk production available upon request.
Target Strategy
Engineered for high binding specificity to IL-6 protein epitopes
Minimal cross-reactivity with related cytokines
Stable binding under physiological conditions
Compatible with biosensor surface functionalization and immunoassay workflows
Product Format
Lyophilized format
Delivered dry for enhanced stability
Reconstitute in PBS or preferred buffer
Solution format
Supplied in PBS-based storage buffer
Typical concentration range 0.5–1 mg/mL
Tagged variants available upon request.
Performance Orientation
Electrochemical biosensors
Cytokine detection assays
Microfluidic and lab-on-chip systems
Surface immobilization workflows
Optical and fluorescence detection platforms
Point-of-care inflammation monitoring research devices
Quality Control
Sequence confirmation
Purity validation (SDS-PAGE / chromatography)
Binding verification testing
Concentration determination
Manufacturing quality review
Certificate of Analysis (CoA) available upon request.
Applications
Inflammation biomarker detection research
Sepsis and infection monitoring studies
Immune response and cytokine signaling research
Immunoassay and biosensor development
Microfluidic diagnostic platform integration
Point-of-care immune monitoring research
Storage
Store at –20 °C for long-term storage or 4 °C for short-term use.
Lyophilized nanobody stable for up to 12 months under recommended conditions. Avoid repeated freeze–thaw cycles.
