Description
The ProbeSeq Tumor Necrosis Factor-Alpha (TNF-α) Nanobody is a recombinant single-domain antibody (VHH) engineered for high-affinity and selective binding to human TNF-α, a central mediator of inflammatory and immune signaling pathways. This nanobody supports research-use assay development, cytokine detection studies, and advanced biosensor integration.
TNF-α is a clinically significant biomarker associated with systemic inflammation, autoimmune disease, infection, and immune activation. Elevated TNF-α levels are widely studied in inflammatory bowel disease, rheumatoid arthritis, sepsis, cancer progression, and host immune response regulation. Sensitive detection of TNF-α is essential for immunology research, therapeutic monitoring, and rapid diagnostic technology development.
Derived from camelid heavy-chain antibody architecture, this nanobody consists of a single monomeric VHH domain (~15 kDa) providing strong antigen recognition, rapid binding kinetics, and exceptional structural stability. Compared with conventional antibodies, nanobodies offer superior thermal stability, reduced steric hindrance, and efficient surface immobilization, enabling high-performance detection in electrochemical biosensors, microfluidic systems, and miniaturized diagnostic platforms.
Typical assay workflows use sample input volumes ranging from approximately 5–50 µL depending on platform configuration and detection method. The nanobody is compatible with serum, plasma, cell culture supernatants, and buffered biological samples following appropriate assay optimization.
What’s Included
Recombinant anti-TNF-α single-domain VHH nanobody
Sequence and expression documentation for research traceability
Reconstitution and handling guidelines
Custom affinity tags (His-tag, biotin, Fc fusion), labeling options, and bulk production available upon request
Target Strategy
Engineered for high binding specificity to TNF-α protein epitopes
Minimal cross-reactivity with related cytokines
Stable binding under physiological conditions
Compatible with biosensor surface functionalization and immunoassay workflows
Product Format
Lyophilized format
Delivered dry for enhanced stability
Reconstitute in PBS or preferred buffer
Solution format
Supplied in PBS-based storage buffer
Typical concentration range 0.5–1 mg/mL
Tagged variants available upon request
Performance Orientation
Electrochemical biosensors
Cytokine detection assays
Microfluidic and lab-on-chip systems
Surface immobilization workflows
Optical and fluorescence detection platforms
Point-of-care inflammation monitoring research devices
Quality Control
Sequence confirmation
Purity validation (SDS-PAGE / chromatography)
Binding verification testing
Concentration determination
Manufacturing quality review
Certificate of Analysis (CoA) available upon request
Applications
Inflammation biomarker detection research
Autoimmune disease studies
Sepsis and infection monitoring research
Immune signaling research
Immunoassay and biosensor development
Microfluidic diagnostic platform integration
Storage
Store at –20 °C for long-term storage or 4 °C for short-term use.
Lyophilized nanobody stable for up to 12 months under recommended conditions. Avoid repeated freeze–thaw cycles.
