Description
The ProbeSeq Vascular Endothelial Growth Factor (VEGF) Nanobody is a recombinant single-domain antibody (VHH) engineered for high-affinity and selective binding to human VEGF, a secreted signaling protein that regulates angiogenesis, vascular permeability, and endothelial cell growth. This nanobody supports research-use diagnostic assay development, angiogenesis studies, and advanced biosensor integration.
VEGF is a clinically significant biomarker associated with tumor progression, cancer metastasis, cardiovascular disease, and tissue regeneration. Elevated VEGF levels are widely studied in oncology, ophthalmology, and vascular biology research. Sensitive detection of VEGF is critical for tumor microenvironment studies, therapeutic monitoring, and development of rapid diagnostic technologies.
Derived from camelid heavy-chain antibody architecture, this nanobody consists of a single monomeric VHH domain (~15 kDa) providing strong antigen recognition, rapid binding kinetics, and exceptional structural stability. Compared with conventional antibodies, nanobodies offer superior thermal stability, efficient surface immobilization, and reduced steric hindrance, enabling high-performance detection in electrochemical biosensors, microfluidic platforms, and miniaturized diagnostic systems.
Typical assay workflows use sample input volumes ranging from approximately 5–50 µL depending on platform configuration and detection method. The nanobody is compatible with serum, plasma, cell culture supernatants, extracellular vesicles, and buffered biological samples following appropriate assay optimization.
What’s Included
Recombinant anti-VEGF single-domain VHH nanobody
Sequence and expression documentation for research traceability
Reconstitution and handling guidelines
Custom affinity tags (His-tag, biotin, Fc fusion), labeling options, and bulk production available upon request
Target Strategy
Engineered for high binding specificity to VEGF protein epitopes
Minimal cross-reactivity with related growth factors
Stable binding under physiological conditions
Compatible with biosensor surface functionalization and immunoassay workflows
Product Format
Lyophilized format
Delivered dry for enhanced stability
Reconstitute in PBS or preferred buffer
Solution format
Supplied in PBS-based storage buffer
Typical concentration range 0.5–1 mg/mL
Tagged variants available upon request
Performance Orientation
Electrochemical biosensors
Angiogenesis and oncology detection assays
Microfluidic and lab-on-chip systems
Surface immobilization workflows
Optical and fluorescence detection platforms
Point-of-care diagnostic research devices
Quality Control
Sequence confirmation
Purity validation (SDS-PAGE / chromatography)
Binding verification testing
Concentration determination
Manufacturing quality review
Certificate of Analysis (CoA) available upon request
Applications
Angiogenesis research
Cancer progression and tumor biology studies
Cardiovascular and vascular biology research
Immunoassay and biosensor development
Microfluidic diagnostic platform integration
Point-of-care biomarker detection research
Storage
Store at –20 °C for long-term storage or 4 °C for short-term use.
Lyophilized nanobody stable for up to 12 months under recommended conditions. Avoid repeated freeze–thaw cycles.
