Description
The ProbeSeq Alpha-Fetoprotein (AFP) mRNA LAMP Primer Set is a ready-to-use six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting AFP transcripts. This product supports research-use molecular oncology applications, including hepatocellular carcinoma (HCC) studies, tumor marker expression analysis, and integration into rapid isothermal amplification platforms.
AFP is a widely studied biomarker associated with hepatocellular carcinoma and certain germ cell tumors. While traditionally detected at the protein level, nucleic acid–based detection of AFP transcripts is increasingly relevant for translational oncology research, circulating tumor RNA studies, and rapid molecular assay development.
This primer system is structured to promote stable amplification kinetics under isothermal conditions while supporting reliable fluorescence signal generation. Primer architecture is optimized to reduce non-specific amplification artifacts and to support compatibility with fluorescence-based monitoring systems.
What’s Included
Each set contains a complete LAMP primer architecture:
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F3 and B3 outer primers
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FIP and BIP inner primers
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LF and LB loop primers
Primers are individually labeled and supplied with sequence documentation for research traceability and workflow integration.
Target Strategy
The primer system targets conserved regions within AFP transcripts selected for:
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Transcript specificity
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Minimal homology to unrelated human sequences
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Compatibility with reverse transcription–LAMP (RT-LAMP) workflows
Target selection prioritizes assay robustness and integration into fluorescence-based detection systems.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• 100 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer
Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom bulk quantities are available for larger translational research programs.
Performance Orientation
Designed for compatibility with:
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Reverse transcription–LAMP (RT-LAMP) workflows
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Intercalating dye fluorescence monitoring
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Real-time isothermal-capable instruments
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Portable fluorescence readers
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Microfluidic and cartridge-integrated amplification systems
Typical reaction temperature range: 60–65 °C. RNA-based applications require inclusion of reverse transcriptase activity in the reaction system.
Quality Control
Each batch undergoes:
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Sequence verification
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Mass confirmation
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Storage
Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended conditions.
Avoid repeated freeze–thaw cycles for solution format.
Applications
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Hepatocellular carcinoma research
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Tumor marker transcript detection studies
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Circulating tumor RNA assay development
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Rapid isothermal amplification prototyping
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Biosensor and microfluidic oncology integration research
