Description
The ProbeSeq Epithelial Cell Adhesion Molecule (EpCAM) mRNA LAMP Primer Set is a ready-to-use six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting EpCAM transcripts. This product supports research-use molecular oncology applications, including circulating tumor cell (CTC) research, epithelial tumor biology studies, and integration into rapid isothermal amplification platforms.
EpCAM is a transmembrane glycoprotein overexpressed in a wide range of epithelial-derived malignancies, including breast, colorectal, pancreatic, and lung cancers. It is widely used as a marker for circulating tumor cell detection and tumor burden research. Nucleic acid–based detection of EpCAM transcripts is increasingly relevant for translational oncology workflows and liquid biopsy assay development.
This primer system is structured to promote stable amplification kinetics under isothermal conditions while enabling reliable fluorescence signal development. Primer architecture is optimized to minimize non-specific amplification artifacts and ensure compatibility with fluorescence-based monitoring systems.
What’s Included
Each set contains a complete LAMP primer architecture:
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F3 and B3 outer primers
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FIP and BIP inner primers
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LF and LB loop primers
Primers are individually labeled and supplied with sequence documentation for research traceability and workflow integration.
Target Strategy
The primer system targets conserved regions within EpCAM transcripts selected for:
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Transcript specificity
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Minimal homology to non-target sequences
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Compatibility with reverse transcription–LAMP (RT-LAMP) workflows
Target selection prioritizes assay robustness and integration into fluorescence-based detection systems.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• 100 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer
Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom bulk quantities are available for translational oncology programs.
Performance Orientation
Designed for compatibility with:
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Reverse transcription–LAMP (RT-LAMP) workflows
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Intercalating dye fluorescence monitoring
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Real-time isothermal-capable instruments
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Portable fluorescence readers
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Microfluidic and cartridge-integrated amplification systems
Typical reaction temperature range: 60–65 °C. RNA-based applications require inclusion of reverse transcriptase activity in the reaction system.
Quality Control
Each batch undergoes:
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Sequence verification
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Mass confirmation
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Storage
Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended conditions.
Avoid repeated freeze–thaw cycles for solution format.
Applications
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Circulating tumor cell (CTC) research
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Epithelial cancer molecular studies
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Liquid biopsy assay development
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Rapid isothermal amplification prototyping
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Biosensor and microfluidic oncology integration research
Important Notice
For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.
