Description
The ProbeSeq Hepatitis B Virus (HBV) LAMP Primer Set is a ready-to-use six-primer system engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting conserved genomic regions of HBV. This product supports research-use molecular detection, assay development, and rapid prototyping of isothermal amplification platforms.
Hepatitis B Virus remains a major global health concern and a key target in molecular diagnostics research, particularly in efforts to develop rapid and decentralized nucleic acid detection systems. LAMP enables amplification at a constant temperature, simplifying instrumentation and reducing time-to-result compared to conventional PCR workflows. Reliable amplification performance depends heavily on primer architecture; this primer set has been structured to promote stable amplification kinetics and consistent fluorescence signal generation while minimizing non-specific amplification artifacts.
What’s Included
Each set contains a complete LAMP primer architecture:
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F3 and B3 outer primers
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FIP and BIP inner primers
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LF and LB loop primers
Primers are individually labeled and supplied with sequence documentation for traceability and research integration.
Target Strategy
The primer system targets a conserved region of the HBV genome selected for:
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Broad genotype coverage
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Minimal homology to human genomic sequences
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Reduced risk of non-specific amplification
Target selection prioritizes robustness and compatibility with fluorescence-based detection workflows.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• 100 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer
Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom bulk quantities are available for larger research or device-development programs.
Performance Orientation
Designed for compatibility with fluorescence-based LAMP workflows, including:
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Intercalating dye fluorescence monitoring
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Real-time isothermal-capable instruments
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Portable fluorescence readers
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Microfluidic and cartridge-integrated systems
Typical reaction temperature range: 60–65 °C. Primer ratios follow standard LAMP architecture; optimization may be required depending on enzyme system and reaction chemistry.
Quality Control
Each batch undergoes:
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Sequence verification
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Mass confirmation
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Storage
Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended storage conditions.
Avoid repeated freeze–thaw cycles for solution format.
Applications
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HBV molecular detection research
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Rapid isothermal assay development
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Blood-borne pathogen research panels
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Point-of-care testing platform prototyping
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Biosensor and microfluidic integration studies
Important Notice
For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.
