Description
The ProbeSeq EGFR Mutation Panel LAMP Primer Set is a mutation-targeted six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) of key EGFR hotspot variants. This panel supports research-use molecular oncology applications, including lung cancer mutation research, liquid biopsy assay development, and decentralized isothermal amplification platform integration.
EGFR mutations such as L858R, T790M, and exon 19 deletions are among the most clinically relevant oncogenic drivers in non-small cell lung cancer (NSCLC). Rapid and selective detection of these variants is critical in translational oncology research and therapeutic resistance studies. Isothermal amplification technologies such as LAMP provide a simplified alternative to PCR-based workflows, enabling mutation detection without thermocycling instrumentation.
This primer panel is structured to promote selective amplification of target mutant sequences under isothermal conditions, supporting fluorescence-based signal monitoring while reducing amplification of wild-type background sequence.
What’s Included
Each mutation configuration contains a complete LAMP primer architecture:
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F3 and B3 outer primers
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FIP and BIP inner primers
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LF and LB loop primers
Available formats:
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Individual mutation-specific sets (L858R, T790M, or exon 19 deletion)
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Combined multi-mutation panel
Primers are individually labeled and supplied with sequence documentation for research traceability.
Target Strategy
The primer architecture is designed to support:
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Preferential amplification of selected EGFR mutant variants
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Reduced amplification of wild-type EGFR sequence
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Compatibility with fluorescence-based detection systems
Target regions are selected based on mutation frequency and translational oncology relevance.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced long-term stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• 100 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer
Standard packaging supports approximately 100–200 reactions per mutation set depending on reaction volume and optimization parameters. Custom panel configurations are available upon request.
Performance Orientation
Designed for compatibility with:
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Intercalating dye fluorescence monitoring
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Real-time isothermal-capable instruments
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Portable fluorescence readers
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Microfluidic or cartridge-integrated amplification platforms
Typical reaction temperature range: 60–65 °C. Mutation discrimination performance may require optimization depending on enzyme system, template concentration, and assay design.
Quality Control
Each batch undergoes:
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Sequence verification
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Mass confirmation
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Storage
Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended conditions.
Avoid repeated freeze–thaw cycles for solution format.
Applications
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EGFR mutation research
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Non-small cell lung cancer (NSCLC) molecular studies
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Therapeutic resistance research (T790M)
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Liquid biopsy assay development
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Rapid isothermal mutation detection prototyping
Important Notice
For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.
