B-Type Natriuretic Peptide (BNP / NPPB) mRNA LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

B-Type Natriuretic Peptide (BNP / NPPB) mRNA LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

$2,415.00

Pre-optimized LAMP primer system targeting B-type Natriuretic Peptide (NPPB) transcripts for fluorescence-based isothermal amplification. Designed for research-use cardiac stress and heart failure studies and integration into rapid isothermal assay platforms. For Research Use Only (RUO).

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Description

The ProbeSeq B-Type Natriuretic Peptide (BNP / NPPB) mRNA LAMP Primer Set is a ready-to-use six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting NPPB transcripts. This product supports research-use molecular cardiology applications, including heart failure research, myocardial stress studies, and integration into rapid isothermal amplification systems.

BNP (encoded by the NPPB gene) is a cardiac hormone released in response to ventricular stretch and increased wall tension. It is widely studied as a biomarker of heart failure, cardiac remodeling, and hemodynamic stress. Transcript-level detection of NPPB provides opportunities for mechanistic cardiac research, gene expression profiling, and experimental cardiovascular model systems.

This primer system is structured to promote stable amplification kinetics under isothermal conditions while enabling consistent fluorescence signal generation. Primer architecture is optimized to minimize non-specific amplification artifacts and to support reliable assay development workflows.

What’s Included

Each set contains a complete LAMP primer architecture:

  • F3 and B3 outer primers

  • FIP and BIP inner primers

  • LF and LB loop primers

Primers are individually labeled and supplied with sequence documentation for research traceability and workflow integration.

Target Strategy

The primer system targets conserved regions within NPPB transcripts selected for:

  • Cardiac transcript specificity

  • Minimal homology to non-target human genes

  • Compatibility with reverse transcription–LAMP (RT-LAMP) workflows

Target selection prioritizes assay robustness and compatibility with fluorescence-based monitoring systems.

Product Format

Available in two configurations:

Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer

Solution format
• 500 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer

Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom bulk quantities are available for cardiovascular research programs.

Performance Orientation

Designed for compatibility with:

  • Reverse transcription–LAMP (RT-LAMP) workflows

  • Intercalating dye fluorescence monitoring

  • Real-time isothermal-capable instruments

  • Portable fluorescence readers

  • Microfluidic and cartridge-integrated amplification systems

Typical reaction temperature range: 60–65 °C. RNA-based applications require inclusion of reverse transcriptase activity in the reaction system.

Quality Control

Each batch undergoes:

  • Sequence verification

  • Mass confirmation

  • Concentration validation

  • Manufacturing quality control review

Certificate of Analysis (CoA) available upon request.

Storage

Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended conditions.
Avoid repeated freeze–thaw cycles for solution format.

Applications

  • Heart failure research

  • Ventricular stress and remodeling studies

  • Molecular cardiology research

  • Rapid isothermal assay prototyping

  • Biosensor and microfluidic cardiovascular integration research

Important Notice

For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.

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