HIV-1 LAMP Primer Set (Fluorescent Detection, RUO)

HIV-1 LAMP Primer Set (Fluorescent Detection, RUO)

$1,830.00

Pre-designed and quality-controlled LAMP primer set targeting conserved regions of HIV-1 for fluorescence-based isothermal amplification. Supplied lyophilized or in nuclease-free solution, optimized for real-time monitoring and research-use molecular assay development.

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Description

The ProbeSeq HIV-1 LAMP Primer Set is a ready-to-use six-primer architecture engineered for rapid isothermal amplification of HIV-1 nucleic acids under fluorescence monitoring. Designed for researchers developing molecular assays, portable diagnostics, and biosensing systems, this product eliminates the need for in-house primer design and accelerates assay prototyping.

Unlike generic oligo offerings, this primer system is structured to support stable amplification kinetics and controlled fluorescence trajectory, reducing non-specific amplification artifacts commonly encountered in LAMP workflows.

Whatโ€™s Included

Each set contains a complete LAMP architecture:

  • F3 and B3 outer primers

  • FIP and BIP inner primers

  • LF and LB loop primers

Primers are individually labeled and supplied with sequence documentation.

Target Strategy

The primer system targets a conserved HIV-1 genomic region selected for:

  • Broad subtype coverage

  • Minimal homology to human sequences

  • Reduced risk of non-specific amplification

Target selection prioritizes research robustness and assay reproducibility.

Product Format

Available in two configurations:

Lyophilized format
โ€ข Delivered dry for maximum stability
โ€ข Reconstitute in nuclease-free water or buffer

Solution format
โ€ข 500 ยตM stock concentration per primer
โ€ข Supplied in nuclease-free water or TE buffer

Standard package supports approximately 100โ€“200 reactions depending on final reaction volume.

Custom synthesis scales are available for bulk or device-integration projects.

Performance Orientation

Designed for fluorescence-based LAMP systems including:

  • Intercalating dye monitoring

  • Real-time isothermal fluorescence instruments

  • Portable readers

  • Microfluidic cartridge platforms

Typical reaction temperature range: 60โ€“65 ยฐC
Primer ratios follow standard LAMP architecture; optimization may be performed based on enzyme system.

Quality Control

Each batch undergoes:

  • Sequence confirmation

  • Mass verification

  • Concentration validation

  • Manufacturing QC documentation

Certificate of Analysis available upon request.

Storage

Store at โ€“20 ยฐC.
Lyophilized primers remain stable for 12 months under recommended conditions.
Avoid repeated freezeโ€“thaw cycles for solution format.

Applications

  • HIV molecular detection research

  • Rapid isothermal assay development

  • Point-of-care platform prototyping

  • Electrochemical and fluorescence biosensor integration

  • Academic and translational research studies

Important Notice

For Research Use Only (RUO).
Not for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.

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