Description
The ProbeSeq Influenza A LAMP Primer Set is a ready-to-use six-primer system engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting conserved regions of Influenza A virus (IAV). This product is designed for research-use molecular detection, assay development, and rapid prototyping of isothermal diagnostic platforms.
Influenza A remains a major cause of seasonal respiratory illness and pandemic outbreaks, making it a high-priority target for rapid nucleic acid detection research. Isothermal amplification technologies such as LAMP enable nucleic acid amplification at a constant temperature, eliminating the need for thermocycling and supporting simplified instrumentation and decentralized testing concepts. Reliable LAMP performance depends on carefully balanced primer architecture; this primer set has been optimized to promote robust amplification kinetics and stable fluorescence signal development while minimizing non-specific amplification artifacts.
What’s Included
Each set contains a complete LAMP primer architecture:
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F3 and B3 outer primers
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FIP and BIP inner primers
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LF and LB loop primers
Primers are individually labeled and supplied with sequence documentation for traceability and integration into research workflows.
Target Strategy
The primer system targets a conserved genomic region of Influenza A selected for:
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Broad subtype coverage across circulating strains
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Minimal homology to human genomic sequences
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Reduced risk of non-specific amplification
Target selection prioritizes assay robustness and compatibility with research-based fluorescence monitoring systems.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for maximum stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• 500 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer
Standard package supports approximately 100–200 reactions depending on reaction volume and optimization conditions. Custom synthesis scales are available for bulk research or device-integration projects.
Performance Orientation
Designed for compatibility with fluorescence-based LAMP workflows, including:
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Intercalating dye–based fluorescence monitoring
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Real-time isothermal-capable qPCR instruments
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Portable fluorescence readers
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Microfluidic and cartridge-integrated amplification systems
Typical reaction temperature range: 60–65 °C. Primer ratios follow standard LAMP architecture; optimization may be performed based on enzyme system and detection chemistry.
Quality Control
Each batch undergoes:
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Sequence verification
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Mass confirmation
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Storage
Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended storage conditions.
Avoid repeated freeze–thaw cycles for solution format.
Applications
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Influenza A molecular detection research
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Rapid isothermal amplification assay development
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Respiratory pathogen research panels
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Point-of-care platform prototyping
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Fluorescence-based biosensor integration
Important Notice
For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.
