Hepatitis C Virus (HCV) LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

Hepatitis C Virus (HCV) LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

$2,900.00

Pre-optimized LAMP primer system targeting conserved regions of Hepatitis C Virus (HCV) for fluorescence-based isothermal amplification. Supplied lyophilized or in nuclease-free solution, designed for rapid nucleic acid detection research and integration into isothermal and point-of-care assay platforms. For Research Use Only (RUO).

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Description

The ProbeSeq Hepatitis C Virus (HCV) LAMP Primer Set is a ready-to-use six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting conserved genomic regions of HCV. This product is intended for research-use molecular detection, rapid assay development, and integration into decentralized nucleic acid amplification platforms.

HCV remains a major blood-borne pathogen of global health significance and a key target for molecular detection research, particularly in the development of rapid, simplified testing technologies. LAMP enables nucleic acid amplification at a constant temperature, eliminating thermocycling requirements and supporting portable, low-infrastructure assay concepts. Amplification performance in LAMP systems depends strongly on primer design and structural balance; this primer set has been configured to support stable amplification kinetics and reliable fluorescence signal generation while minimizing non-specific amplification artifacts.

What’s Included

Each set contains a complete LAMP primer architecture:

  • F3 and B3 outer primers

  • FIP and BIP inner primers

  • LF and LB loop primers

Primers are individually labeled and supplied with sequence documentation for workflow traceability and research integration.

Target Strategy

The primer system targets a conserved region of the HCV genome selected for:

  • Broad genotype coverage

  • Reduced homology to human genomic sequences

  • Enhanced specificity within amplification conditions

Target selection prioritizes research robustness and compatibility with fluorescence-based monitoring systems.

Product Format

Available in two configurations:

Lyophilized format
• Delivered dry for enhanced long-term stability
• Reconstitute in nuclease-free water or preferred buffer

Solution format
• 100 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer

Standard packaging supports approximately 100–200 reactions depending on final reaction volume and optimization parameters. Custom synthesis scales are available for larger research or device-development programs.

Performance Orientation

Designed for compatibility with fluorescence-based LAMP workflows, including:

  • Intercalating dye fluorescence monitoring

  • Real-time isothermal-capable instruments

  • Portable fluorescence readers

  • Cartridge-integrated and microfluidic amplification systems

Typical reaction temperature range: 60–65 °C. Primer ratios follow standard LAMP architecture; optimization may be required depending on enzyme chemistry and detection system.

Quality Control

Each batch undergoes:

  • Sequence verification

  • Mass confirmation

  • Concentration validation

  • Manufacturing quality control review

Certificate of Analysis (CoA) available upon request.

Storage

Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended storage conditions.
Avoid repeated freeze–thaw cycles for solution format.

Applications

  • HCV molecular detection research

  • Rapid isothermal amplification assay development

  • Blood-borne pathogen research panels

  • Point-of-care testing platform prototyping

  • Biosensor and microfluidic integration studies

Important Notice

For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.

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