Prostate-Specific Antigen (PSA) mRNA LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

Prostate-Specific Antigen (PSA) mRNA LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

$2,415.00

Pre-optimized LAMP primer system targeting Prostate-Specific Antigen (PSA/KLK3) transcripts for fluorescence-based isothermal amplification. Designed for research-use molecular oncology applications including prostate cancer studies and rapid isothermal assay platform integration. For Research Use Only (RUO).

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Description

The ProbeSeq Prostate-Specific Antigen (PSA) mRNA LAMP Primer Set is a ready-to-use six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting KLK3 (PSA) transcripts. This product supports research-use molecular oncology applications, including prostate cancer molecular studies, circulating tumor RNA research, and integration into rapid isothermal amplification platforms.

PSA (encoded by KLK3) is a widely studied biomarker associated with prostate cancer progression and monitoring. While commonly measured at the protein level, nucleic acid–based detection of PSA transcripts is increasingly relevant for translational oncology research, circulating tumor cell studies, and decentralized molecular testing concepts.

This primer system is structured to promote stable amplification kinetics under isothermal conditions while supporting reliable fluorescence signal development. Primer architecture is optimized to minimize non-specific amplification artifacts and ensure compatibility with fluorescence-based monitoring systems.

What’s Included

Each set contains a complete LAMP primer architecture:

  • F3 and B3 outer primers

  • FIP and BIP inner primers

  • LF and LB loop primers

Primers are individually labeled and supplied with sequence documentation for research traceability and workflow integration.

Target Strategy

The primer system targets conserved regions within KLK3 (PSA) transcripts selected for:

  • Transcript specificity

  • Minimal homology to non-target sequences

  • Compatibility with reverse transcription–LAMP (RT-LAMP) workflows

Target selection prioritizes assay robustness and integration into fluorescence-based detection systems.

Product Format

Available in two configurations:

Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer

Solution format
• 500 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer

Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom bulk quantities are available for larger translational research programs.

Performance Orientation

Designed for compatibility with:

  • Reverse transcription–LAMP (RT-LAMP) workflows

  • Intercalating dye fluorescence monitoring

  • Real-time isothermal-capable instruments

  • Portable fluorescence readers

  • Microfluidic and cartridge-integrated amplification systems

Typical reaction temperature range: 60–65 °C. RNA-based applications require inclusion of reverse transcriptase activity in the reaction system.

Quality Control

Each batch undergoes:

  • Sequence verification

  • Mass confirmation

  • Concentration validation

  • Manufacturing quality control review

Certificate of Analysis (CoA) available upon request.

Storage

Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended conditions.
Avoid repeated freeze–thaw cycles for solution format.

Applications

  • Prostate cancer molecular research

  • Tumor marker transcript detection studies

  • Circulating tumor RNA assay development

  • Rapid isothermal amplification prototyping

  • Biosensor and microfluidic oncology integration research

Important Notice

For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.

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