Troponin T (TNNT2) mRNA LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

Troponin T (TNNT2) mRNA LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

$2,415.00

Pre-optimized LAMP primer system targeting cardiac Troponin T (TNNT2) transcripts for fluorescence-based isothermal amplification. Designed for research-use cardiovascular and myocardial injury studies and integration into rapid isothermal assay platforms. For Research Use Only (RUO).

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Description

The ProbeSeq Troponin T (TNNT2) mRNA LAMP Primer Set is a ready-to-use six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting TNNT2 transcripts. This product supports research-use molecular cardiology applications, including myocardial injury studies, cardiac transcript profiling, and integration into rapid isothermal amplification systems.

Cardiac Troponin T (TNNT2) is a central component of the troponin complex involved in myocardial contraction and is widely studied as a biomarker of cardiac injury and stress. While traditionally detected at the protein level, transcript-level detection offers additional insights in experimental cardiac models, ischemia research, and molecular pathway studies. LAMP enables nucleic acid amplification under constant temperature conditions, reducing instrumentation complexity and supporting simplified and portable workflow concepts.

This primer system is structured to promote stable amplification kinetics and reliable fluorescence signal generation while minimizing non-specific amplification artifacts.

What’s Included

Each set contains a complete LAMP primer architecture:

  • F3 and B3 outer primers

  • FIP and BIP inner primers

  • LF and LB loop primers

Primers are individually labeled and supplied with sequence documentation for research traceability and workflow integration.

Target Strategy

The primer system targets conserved regions within TNNT2 transcripts selected for:

  • Cardiac transcript specificity

  • Minimal homology to non-target human genes

  • Compatibility with reverse transcription–LAMP (RT-LAMP) workflows

Target selection prioritizes assay robustness and compatibility with fluorescence-based monitoring systems.

Product Format

Available in two configurations:

Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer

Solution format
• 100 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer

Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom bulk quantities are available for cardiovascular research programs.

Performance Orientation

Designed for compatibility with:

  • Reverse transcription–LAMP (RT-LAMP) workflows

  • Intercalating dye fluorescence monitoring

  • Real-time isothermal-capable instruments

  • Portable fluorescence readers

  • Microfluidic and cartridge-integrated amplification systems

Typical reaction temperature range: 60–65 °C. RNA-based applications require inclusion of reverse transcriptase activity in the reaction system.

Quality Control

Each batch undergoes:

  • Sequence verification

  • Mass confirmation

  • Concentration validation

  • Manufacturing quality control review

Certificate of Analysis (CoA) available upon request.

Storage

Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended conditions.
Avoid repeated freeze–thaw cycles for solution format.

Applications

  • Myocardial injury research

  • Ischemia and cardiac stress studies

  • Molecular cardiology research

  • Rapid isothermal assay prototyping

  • Biosensor and microfluidic cardiovascular integration research

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