Description
The ProbeSeq HER2 (ERBB2) Amplification LAMP Primer Set is a ready-to-use six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting genomic regions associated with ERBB2 (HER2) amplification. This product supports research-use molecular oncology applications, including HER2 copy number research, translational cancer studies, and integration into isothermal amplification platforms.
HER2 (ERBB2) amplification is a clinically significant genomic alteration observed in breast, gastric, and other solid tumors. Detection of HER2 copy number changes plays a critical role in oncology research, therapeutic stratification studies, and biomarker development. Isothermal amplification technologies such as LAMP offer simplified workflows compared to conventional PCR-based methods and enable rapid amplification under constant temperature conditions.
This primer system is structured to support amplification of ERBB2 genomic targets associated with amplification events, enabling fluorescence-based detection while maintaining stable amplification kinetics and minimizing non-specific signal generation.
What’s Included
Each set contains a complete LAMP primer architecture:
-
F3 and B3 outer primers
-
FIP and BIP inner primers
-
LF and LB loop primers
Primers are individually labeled and supplied with sequence documentation for research traceability and workflow integration.
Target Strategy
The primer system is designed to support:
-
Detection of ERBB2 genomic regions relevant to amplification assessment
-
Compatibility with relative copy number comparison workflows
-
Fluorescence-based monitoring under isothermal conditions
Target regions are selected to align with commonly studied HER2 amplification loci in translational oncology research.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced long-term stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• 500 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer
Standard packaging supports approximately 100–200 reactions depending on reaction volume and experimental design. Custom panel configurations are available for comparative amplification studies.
Performance Orientation
Designed for compatibility with:
-
Intercalating dye fluorescence monitoring
-
Real-time isothermal-capable instruments
-
Portable fluorescence readers
-
Microfluidic or cartridge-integrated amplification systems
Typical reaction temperature range: 60–65 °C. Copy number–oriented applications may require inclusion of reference gene controls and optimization based on template input.
Quality Control
Each batch undergoes:
-
Sequence verification
-
Mass confirmation
-
Concentration validation
-
Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Storage
Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended storage conditions.
Avoid repeated freeze–thaw cycles for solution format.
Applications
-
HER2 amplification research
-
Breast and gastric cancer molecular studies
-
Copy number variation assay development
-
Oncology biomarker platform prototyping
-
Biosensor and microfluidic integration research
Important Notice
For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.
