IDH1 (R132H) Mutation LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

IDH1 (R132H) Mutation LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

$2,850.00

Mutation-focused LAMP primer system targeting the IDH1 R132H hotspot variant for fluorescence-based isothermal amplification. Designed for research-use detection of oncogenic IDH1 mutations and integration into molecular oncology, liquid biopsy, and rapid isothermal assay platforms. For Research Use Only (RUO).

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Description

The ProbeSeq IDH1 (R132H) Mutation LAMP Primer Set is a mutation-specific six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) of the IDH1 R132H hotspot variant. This product supports research-use molecular oncology applications, including glioma mutation research, liquid biopsy assay development, and decentralized isothermal amplification platform integration.

The IDH1 R132H mutation is one of the most clinically significant alterations in diffuse gliomas and is widely used in molecular tumor classification and translational research studies. Rapid mutation-specific detection technologies are increasingly valuable for streamlined research workflows and portable molecular testing concepts.

This primer system is designed to preferentially amplify the R132H mutant sequence under isothermal conditions, enabling fluorescence-based detection without thermocycling instrumentation. Primer architecture has been optimized to support selective amplification kinetics and stable fluorescence signal generation while minimizing amplification of wild-type background sequence.

What’s Included

Each set contains a complete mutation-focused LAMP primer architecture:

  • F3 and B3 outer primers

  • FIP and BIP inner primers

  • LF and LB loop primers

Primers are individually labeled and supplied with sequence documentation for research traceability.

Target Strategy

The primer system is structured to support:

  • Selective amplification of the IDH1 R132H variant

  • Reduced amplification of wild-type IDH1 sequence

  • Compatibility with fluorescence-based monitoring systems

Target region selection is based on high mutation prevalence in glioma and established translational oncology relevance.

Product Format

Available in two configurations:

Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer

Solution format
• 500 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer

Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom mutation configurations available upon request.

Performance Orientation

Designed for compatibility with:

  • Intercalating dye fluorescence monitoring

  • Real-time isothermal-capable instruments

  • Portable fluorescence readers

  • Microfluidic or cartridge-integrated amplification platforms

Typical reaction temperature range: 60–65 °C. Mutation discrimination performance may require optimization depending on enzyme system and template concentration.

Quality Control

Each batch undergoes:

  • Sequence verification

  • Mass confirmation

  • Concentration validation

  • Manufacturing quality control review

Certificate of Analysis (CoA) available upon request.

Storage

Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended conditions.
Avoid repeated freeze–thaw cycles for solution format.

Applications

  • Glioma mutation research

  • Neuro-oncology molecular studies

  • Liquid biopsy assay development

  • Rapid isothermal amplification system prototyping

  • Biosensor and microfluidic integration research

Important Notice

For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.

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