Influenza B LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

Influenza B LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

$2,415.00

Pre-optimized LAMP primer system targeting conserved regions of Influenza B virus for fluorescence-based isothermal amplification. Supplied lyophilized or in nuclease-free solution, designed for rapid respiratory pathogen detection research and integration into isothermal and point-of-care assay platforms. For Research Use Only (RUO).

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Description

The ProbeSeq Influenza B LAMP Primer Set is a ready-to-use six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting conserved genomic regions of Influenza B virus. This product supports research-use molecular detection, respiratory assay development, and rapid isothermal amplification platform prototyping.

Influenza B contributes significantly to seasonal respiratory disease burden and is frequently included in multiplex respiratory testing panels. Rapid and simplified nucleic acid detection methods are essential for translational research and decentralized testing concepts. LAMP enables amplification at a constant temperature, eliminating the need for thermocycling instrumentation while supporting fast time-to-result workflows. Primer architecture has been optimized to promote stable amplification kinetics and reliable fluorescence signal development while minimizing non-specific amplification artifacts.

What’s Included

Each set contains a complete LAMP primer architecture:

  • F3 and B3 outer primers

  • FIP and BIP inner primers

  • LF and LB loop primers

Primers are individually labeled and supplied with sequence documentation for research traceability and seamless workflow integration.

Target Strategy

The primer system targets a conserved genomic region selected for:

  • Broad strain coverage

  • Minimal homology to human genomic sequences

  • Reduced likelihood of cross-reactivity with Influenza A

Target selection prioritizes assay robustness and compatibility with fluorescence-based monitoring systems.

Product Format

Available in two configurations:

Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer

Solution format
• 100 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer

Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom bulk quantities are available for larger respiratory panel development programs.

Performance Orientation

Designed for compatibility with fluorescence-based LAMP workflows, including:

  • Intercalating dye fluorescence monitoring

  • Real-time isothermal-capable instruments

  • Portable fluorescence readers

  • Cartridge-integrated and microfluidic amplification systems

Typical reaction temperature range: 60–65 °C. Primer ratios follow standard LAMP architecture; optimization may be required depending on enzyme chemistry and detection platform.

Quality Control

Each batch undergoes:

  • Sequence verification

  • Mass confirmation

  • Concentration validation

  • Manufacturing quality control review

Certificate of Analysis (CoA) available upon request.

Storage

Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended storage conditions.
Avoid repeated freeze–thaw cycles for solution format.

Applications

  • Influenza B molecular detection research

  • Respiratory pathogen panel development

  • Rapid isothermal amplification assay prototyping

  • Point-of-care testing platform development

  • Biosensor and microfluidic integration studies

Important Notice

For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.

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