Description
The ProbeSeq Interleukin-6 (IL-6) mRNA LAMP Primer Set is a ready-to-use six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting IL6 transcripts. This product supports research-use applications in immunology, inflammation biology, oncology, and cytokine signaling research, as well as integration into rapid isothermal amplification systems.
Interleukin-6 (IL-6) is a multifunctional cytokine involved in immune regulation, acute-phase response, chronic inflammation, and tumor microenvironment signaling. Elevated IL-6 expression is widely studied in autoimmune disorders, sepsis models, cancer progression, and inflammatory cardiovascular conditions. Transcript-level detection of IL6 provides molecular insight into cytokine activation pathways and gene expression dynamics in experimental models.
This primer system is structured to promote stable amplification kinetics under isothermal conditions while enabling reliable fluorescence signal generation. Primer architecture is optimized to minimize non-specific amplification artifacts and support reproducible assay development.
What’s Included
Each set contains a complete LAMP primer architecture:
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F3 and B3 outer primers
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FIP and BIP inner primers
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LF and LB loop primers
Primers are individually labeled and supplied with sequence documentation for research traceability and workflow integration.
Target Strategy
The primer system targets conserved regions within IL6 transcripts selected for:
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Transcript specificity
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Minimal homology to non-target human genes
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Compatibility with reverse transcription–LAMP (RT-LAMP) workflows
Target selection prioritizes assay robustness and compatibility with fluorescence-based detection systems.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• 100 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer
Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom bulk quantities are available for translational research programs.
Performance Orientation
Designed for compatibility with:
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Reverse transcription–LAMP (RT-LAMP) workflows
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Intercalating dye fluorescence monitoring
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Real-time isothermal-capable instruments
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Portable fluorescence readers
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Microfluidic and cartridge-integrated amplification systems
Typical reaction temperature range: 60–65 °C. RNA-based applications require inclusion of reverse transcriptase activity in the reaction system.
Quality Control
Each batch undergoes:
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Sequence verification
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Mass confirmation
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Storage
Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended conditions.
Avoid repeated freeze–thaw cycles for solution format.
Applications
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Cytokine signaling research
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Inflammation and immune activation studies
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Tumor microenvironment research
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Sepsis and cytokine storm models
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Rapid isothermal assay prototyping
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Biosensor and microfluidic immunology integration research
Important Notice
For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.
