Description
The ProbeSeq Mycobacterium tuberculosis (TB) LAMP Primer Set is a ready-to-use six-primer system engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting conserved genomic regions within the Mycobacterium tuberculosis complex (MTBC). This product is designed to support research-use molecular detection, rapid assay development, and integration into isothermal amplification platforms.
Tuberculosis remains one of the leading infectious causes of morbidity and mortality worldwide, driving the need for rapid, sensitive nucleic acid detection technologies suitable for decentralized and resource-limited settings. LAMP provides a constant-temperature amplification method that simplifies instrumentation and shortens time-to-result compared to conventional PCR workflows. Reliable performance, however, depends on carefully structured primer architecture. This primer set has been optimized to promote stable amplification kinetics and consistent fluorescence signal generation while minimizing non-specific amplification artifacts.
What’s Included
Each set contains a complete LAMP primer architecture:
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F3 and B3 outer primers
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FIP and BIP inner primers
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LF and LB loop primers
Primers are individually labeled and supplied with sequence documentation to support traceability and seamless integration into research workflows.
Target Strategy
The primer system targets a conserved region within the M. tuberculosis complex selected for:
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High sequence conservation across MTBC strains
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Specificity relative to non-tuberculous mycobacteria
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Reduced likelihood of non-specific amplification
Target selection prioritizes assay robustness and compatibility with fluorescence-based detection systems.
Product Format
Available in two configurations:
Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer
Solution format
• 500 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer
Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom bulk quantities are available for larger research or device-development programs.
Performance Orientation
Designed for compatibility with fluorescence-based LAMP workflows, including:
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Intercalating dye fluorescence monitoring
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Real-time isothermal-capable instruments
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Portable fluorescence readers
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Microfluidic and cartridge-integrated amplification systems
Typical reaction temperature range: 60–65 °C. Primer ratios follow standard LAMP architecture; optimization may be required depending on enzyme system and reaction chemistry.
Quality Control
Each batch undergoes:
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Sequence verification
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Mass confirmation
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Concentration validation
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Manufacturing quality control review
Certificate of Analysis (CoA) available upon request.
Storage
Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended storage conditions.
Avoid repeated freeze–thaw cycles for solution format.
Applications
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Tuberculosis molecular detection research
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Rapid isothermal assay development
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Respiratory pathogen research panels
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Point-of-care testing platform prototyping
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Biosensor and microfluidic integration studies
Important Notice
For Research Use Only (RUO).
Not intended for diagnostic, therapeutic, or clinical applications.
End-user validation is required for performance verification.
