Respiratory Syncytial Virus (RSV A/B) LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

Respiratory Syncytial Virus (RSV A/B) LAMP Primer Set – Fluorescent Isothermal Amplification (RUO)

$2,900.00

Pre-optimized LAMP primer system targeting conserved regions of Respiratory Syncytial Virus (RSV A and B) for fluorescence-based isothermal amplification. Supplied lyophilized or in nuclease-free solution, designed for rapid respiratory pathogen detection research and integration into isothermal and point-of-care assay platforms. For Research Use Only (RUO).

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Description

he ProbeSeq Respiratory Syncytial Virus (RSV A/B) LAMP Primer Set is a ready-to-use six-primer architecture engineered for fluorescence-based loop-mediated isothermal amplification (LAMP) targeting conserved genomic regions of RSV subtypes A and B. This product supports research-use molecular detection, respiratory panel development, and rapid isothermal assay prototyping.

RSV is a major cause of lower respiratory tract infections in infants, elderly populations, and immunocompromised individuals, making it a high-priority target in respiratory diagnostics research. Isothermal amplification technologies such as LAMP enable nucleic acid amplification at a constant temperature, simplifying instrumentation and supporting decentralized and portable testing concepts. Assay reliability depends heavily on primer structure and thermodynamic balance; this primer set has been configured to promote stable amplification kinetics and controlled fluorescence signal generation while minimizing non-specific amplification artifacts.

What’s Included

Each set contains a complete LAMP primer architecture:

  • F3 and B3 outer primers

  • FIP and BIP inner primers

  • LF and LB loop primers

Primers are individually labeled and supplied with sequence documentation for research traceability and workflow integration.

Target Strategy

The primer system targets conserved genomic regions selected for:

  • Coverage across RSV A and RSV B strains

  • Minimal homology to human genomic sequences

  • Reduced risk of non-specific amplification

Target selection prioritizes assay robustness and compatibility with fluorescence-based monitoring systems.

Product Format

Available in two configurations:

Lyophilized format
• Delivered dry for enhanced stability
• Reconstitute in nuclease-free water or preferred buffer

Solution format
• 500 µM stock concentration per primer
• Supplied in nuclease-free water or TE buffer

Standard packaging supports approximately 100–200 reactions depending on reaction volume and optimization parameters. Custom bulk quantities are available for larger respiratory panel development programs.

Performance Orientation

Designed for compatibility with fluorescence-based LAMP workflows, including:

  • Intercalating dye fluorescence monitoring

  • Real-time isothermal-capable instruments

  • Portable fluorescence readers

  • Cartridge-integrated and microfluidic amplification systems

Typical reaction temperature range: 60–65 °C. Primer ratios follow standard LAMP architecture; optimization may be required depending on enzyme chemistry and detection platform.

Quality Control

Each batch undergoes:

  • Sequence verification

  • Mass confirmation

  • Concentration validation

  • Manufacturing quality control review

Certificate of Analysis (CoA) available upon request.

Storage

Store at –20 °C.
Lyophilized primers are stable for up to 12 months under recommended storage conditions.
Avoid repeated freeze–thaw cycles for solution format.

Applications

  • RSV molecular detection research

  • Respiratory pathogen panel development

  • Rapid isothermal amplification assay development

  • Point-of-care testing platform prototyping

  • Biosensor and microfluidic integration studies

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